Anti-ACOT11抗体(ab105509)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ACOT11
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 215-264 (AEKTRVESVE LVLPPHANHQ GNTFGGQIMA WMENVATIAA SRLCRAHPTL) of Human ACOT11 (NP_671517).

  • 阳性对照
    • HepG2 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon

性能

应用

Our Abpromise guarantee covers the use of ab105509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.
  • 组织特异性
    Isoform 1 is predominantly expressed in skeletal muscle, liver, testis, stomach, spleen, lung and brain. Isoform 2 is predominantly expressed in kidney, uterus, hibernoma and white adipose tissue.
  • 序列相似性
    Contains 2 acyl coenzyme A hydrolase domains.
    Contains 1 START domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACO11_HUMAN antibody
    • ACOT 11 antibody
    • ACOT11 antibody
    • Acyl CoA thioester hydrolase 11 antibody
    • Acyl CoA thioesterase 11 antibody
    • Acyl coenzyme A thioesterase 11 antibody
    • Acyl-CoA thioester hydrolase 11 antibody
    • Acyl-CoA thioesterase 11 antibody
    • Acyl-coenzyme A thioesterase 11 antibody
    • Adipose associated thioesterase antibody
    • Adipose-associated thioesterase antibody
    • BFIT 1 antibody
    • BFIT 2 antibody
    • BFIT antibody
    • BFIT1 antibody
    • BFIT2 antibody
    • Brown fat inducible thioesterase antibody
    • Brown fat-inducible thioesterase antibody
    • KIAA0707 antibody
    • OTTHUMP00000046722 antibody
    • StAR related lipid transfer (START) domain containing 14 antibody
    • STARD 14 antibody
    • STARD14 antibody
    • START domain containing 14 antibody
    • THEA antibody
    • THEM 1 antibody
    • THEM1 antibody
    • Thioesterase adipose associated antibody
    • Thioesterase superfamily member 1 antibody
    see all

图片

  • IHC image of ACOT11 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105509, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-ACOT11 antibody (ab105509) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 68 kDa
    Gel concentration: 12%

文献

ab105509 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Aug 23 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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