山羊多克隆抗体to Aconitase 2
Mouse, Rat, Cow, Dog, Pig
, corresponding to internal sequence amino acids 554-566 of Human Aconitase 2 (NP_001089.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
ab99467 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use at an assay dependent concentration.
||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Belongs to the aconitase/IPM isomerase family.
Information by UniProt
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
Western blot - Anti-Aconitase 2 antibody (ab99467)
Anti-Aconitase 2 antibody (ab99467) at 0.01 µg/ml + Human Heart lysates at 35 µg
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab99467)
ab99467 (3.8µg/ml) staining of paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"