Anti-Aconitase 2抗体[6D1BE4] (ab110320)

概述

  • 产品名称
    Anti-Aconitase 2抗体[6D1BE4]
    参阅全部 Aconitase 2 一抗
  • 描述
    小鼠单克隆抗体[6D1BE4] to Aconitase 2
  • 特异性
    This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting.
  • 经测试应用
    适用于: IP, ICC/IF, In-Cell ELISA, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Borvine heart mitochondria

  • 阳性对照
    • HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    图片

    • ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
      Note: the target protein locates to the mitochondria.
    • Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
      Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320.

    • ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

    文献

    ab110320 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for contacting us. Both ACO2 antibodies cross-react with mouse, however ab110320 is more suitable for immunoprecipitation. Ab110321 is a good WB antibody. In this particular case, I would recommend using ab110320 then. I hope this information...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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