Anti-Aconitase 2抗体[6D1BE4] (ab110320)
Key features and details
- Mouse monoclonal [6D1BE4] to Aconitase 2
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
-
产品名称
Anti-Aconitase 2抗体[6D1BE4]
参阅全部 Aconitase 2 一抗 -
描述
小鼠单克隆抗体[6D1BE4] to Aconitase 2 -
宿主
Mouse -
特异性
This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting. -
经测试应用
适用于: IP, ICC/IF, Flow Cytmore details
不适用于: WB -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
-
纯化说明
ab110320 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
6D1BE4 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110320于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use a concentration of 1 µg/ml.
|
|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
---|
IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
通路
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
序列相似性
Belongs to the aconitase/IPM isomerase family. -
细胞定位
Mitochondrion. - Information by UniProt
-
数据库链接
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
- SwissProt: Q99KI0 Mouse
- SwissProt: Q9ER34 Rat
- Unigene: 643610 Human
see all -
别名
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
图片
-
ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: the target protein locates to the mitochondria. -
Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320. -
ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab110320 尚未被引用在任何文献中。