Anti-Aconitase 1抗体(ab88313)

概述

  • 产品名称Anti-Aconitase 1抗体
    参阅全部 Aconitase 1 一抗
  • 描述
    小鼠多克隆抗体to Aconitase 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human Aconitase 1 protein (NP_002188.1).

  • 阳性对照
    • Human liver tissue lysate; Aconitase 1 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 98 kDa.

靶标

  • 功能Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • 序列相似性Belongs to the aconitase/IPM isomerase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACO 1 antibody
    • ACO1 antibody
    • ACOC_HUMAN antibody
    • Aconitase 1 soluble antibody
    • Aconitase antibody
    • Aconitase1 antibody
    • Aconitate hydratase antibody
    • ACONS antibody
    • Citrate hydro lyase antibody
    • Citrate hydro-lyase antibody
    • Cytoplasmic aconitate hydratase antibody
    • Ferritin repressor protein antibody
    • IRE BP 1 antibody
    • IRE-BP 1 antibody
    • IREB 1 antibody
    • IREB1 antibody
    • IREBP antibody
    • IREBP1 antibody
    • Iron regulatory protein 1 antibody
    • Iron responsive element binding protein 1 antibody
    • Iron-responsive element-binding protein 1 antibody
    • IRP 1 antibody
    • IRP1 antibody
    • OTTHUMP00000045233 antibody
    see all

Anti-Aconitase 1 antibody 图像

  • All lanes : Anti-Aconitase 1 antibody (ab88313) at 1 µg/ml

    Lane 1 : Aconitase 1 transfected 293T cell lysate
    Lane 2 : untransfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 98 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Aconitase 1 antibody (ab88313) at 1 µg/ml + human liver tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 98 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab88313 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88313, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Aconitase 1 antibody (ab88313)参考文献

ab88313 has not yet been referenced specifically in any publications.

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