Anti-Acinus抗体(ab7352)
Key features and details
- Rabbit polyclonal to Acinus
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Acinus抗体 -
描述
兔多克隆抗体to Acinus -
宿主
Rabbit -
特异性
The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. The epitope is a region near the C-terminus of the cleaved active peptide p17. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
TRTALHGVKWPQSNPK
, corresponding to amino acids 1065-1080 of Human Acinus or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is locatednear the C-terminus of the cleaved active peptide p17. -
阳性对照
- K562 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified IgG was prepared from whole serum by affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367).
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说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367). |
靶标
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功能
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3. -
组织特异性
Ubiquitous. -
序列相似性
Contains 1 SAP domain. -
翻译后修饰
Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 22985 Human
- Omim: 604562 Human
- SwissProt: Q9UKV3 Human
- Unigene: 124490 Human
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别名
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7352 at 20 µg/ml
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ab7352 at 10µg/ml staining Acinus in K562 cells by ICC/IF
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Anti-Acinus antibody (ab7352) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa -
Immunofluorescence of Acinus in Hela cells using ab7352 at 10 ug/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab7352 尚未被引用在任何文献中。