概述

  • 产品名称Anti-Acidic Cytokeratin抗体[AE1]
    参阅全部 Acidic Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[AE1] to Acidic Cytokeratin
  • 特异性ab80824 recognizes the 56.5, 50, 50’, 48, and 40kDa keratins of the acidic subfamily.
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Human, Monkey
  • 免疫原

    Human epidermal keratin.

  • 阳性对照
    • Skin. Squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab80824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-P
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Acidic Cytokeratin antibody [AE1] 图像

    • Formalin-fixed, paraffin-embedded human squamous cell carcinoma of lung stained with ab80824 at 1/50 dilution using a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

    Anti-Acidic Cytokeratin antibody [AE1] (ab80824)参考文献

    This product has been referenced in:
    • Cooper D  et al. The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation 28:30-5 (1984). Read more (PubMed: 6083891) »
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »

    See all 3 Publications for this product

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