Acid Phosphatase Assay试剂盒(Fluorometric) (ab83370)

概述

  • 产品名称Acid Phosphatase Assay试剂盒(Fluorometric)
    参阅全部 Acid phosphatase 试剂盒
  • 测试
    500 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Enzyme activity
  • 灵敏度
    0.001 mU/ml
  • 产品概述

    Abcam's Acid Phosphatase Assay Kit (Fluorometric) uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The detection sensitivity is ~1 µU, more sensitive than colorimetric assays.

    The kit is suitable for both research and drug discovery.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
  • 细胞定位ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
  • 别名
    • Acid phosphatase 1 soluble
    • Acid phosphatase 2 lysosomal
    • Acid phosphatase 5 tartrate resistant
    • Acid phosphatase of erythrocyte
    • Acid phosphatase prostate
    • ACP1
    • ACP2
    • ACP3
    • ACP5
    • ACPP
    • Adipocyte acid phosphatase
    • HAAP
    • LAP
    • LMW PTP
    • LMW PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • Lysosomal acid phosphatase
    • PAP
    • Prostatic acid phosphotase
    • Red cell acid phosphatase 1
    • Tartrate resistant acid ATPase
    • Tartrate resistant acid phosphatase 5
    • Tartrate resistant acid phosphatase type 5
    • TR AP
    • TRAP
    • TrATPase
    see all

应用

Our Abpromise guarantee covers the use of ab83370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Acid Phosphatase Assay Kit (Fluorometric) 图像

  • 4-Methylumbelliferone (4-MU) Standard Curve.

实验方案

Acid Phosphatase Assay Kit (Fluorometric) (ab83370)参考文献

ab83370 has not yet been referenced specifically in any publications.

Product Wall


For Acid Phosphatase Assay Kit ab83370, the pH of the buffer prevents alkaline phosphatase from working (ALP can only function in an alkaline pH whereas AP works in an acidic pH) so you only measure AP activity.
Acid phosphatase (AP) can depho...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"