Acid Phosphatase Assay试剂盒(Fluorometric) (ab83370)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.001 mU/ml
概述
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产品名称
Acid Phosphatase Assay试剂盒(Fluorometric)
参阅全部 Acid phosphatase 试剂盒 -
检测方法
Fluorescent -
样品类型
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Enzyme activity -
灵敏度
0.001 mU/ml -
产品概述
Abcam's Acid Phosphatase Assay Kit (Fluorometric) uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K421 Acid Phosphatase Activity Fluorometric Assay Kit. K421-500 is the same size as the 500 test size of ab83370.
The detection sensitivity is ~1 µU, more sensitive than colorimetric assays.
The kit is suitable for both research and drug discovery.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests AP Assay buffer 1 x 100ml AP Enzyme (Lyophilised) 1 vial MUP Substrate 1 vial Stop Solution 1 x 25ml -
研究领域
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相关性
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease. -
细胞定位
ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein. -
别名
- Acid phosphatase 1 soluble
- Acid phosphatase 2 lysosomal
- Acid phosphatase 5 tartrate resistant
see all
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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