Anti-Acid Phosphatase抗体(ab58688)

概述

  • 产品名称Anti-Acid Phosphatase抗体
    参阅全部 Acid Phosphatase 一抗
  • 描述
    兔多克隆抗体to Acid Phosphatase
  • 特异性This antibody reacts with erythrocyte acid phosphatase.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Pig
  • 免疫原

    A region within synthetic peptide: SENWRVDSAA TSGYEIGNPP DYRGQSCMKR HGIPMSHVAR QITKEDFATF, corresponding to N terminal amino acids 37-86 of Human Acid Phosphatase

  • 阳性对照
    • Jurkat cell lysate, human kidney tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab58688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

Anti-Acid Phosphatase antibody 图像

  • Anti-Acid Phosphatase antibody (ab58688) at 1.25 µg/ml + Jurkat cell lysate at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 dilution.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Acid Phosphatase with ab58688 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-Acid Phosphatase antibody (ab58688)参考文献

ab58688 has not yet been referenced specifically in any publications.

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