概述

  • 产品名称Anti-Acid Phosphatase抗体
    参阅全部 Acid Phosphatase 一抗
  • 描述
    兔多克隆抗体to Acid Phosphatase
  • 特异性By immunoelectrophoresis, gives a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Potato Acid Phosphatase.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Potato
  • 免疫原

    Potato Acid Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000.
WB 1/2000 - 1/10000.
ELISA 1/4000 - 1/40000.

靶标

  • 功能Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • 组织特异性T-lymphocytes express only isoform 2.
  • 序列相似性Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 别名
    • Acid phosphatase 1 soluble antibody
    • Acid phosphatase of erythrocyte antibody
    • ACP1 antibody
    • Adipocyte acid phosphatase antibody
    • Cytoplasmic phosphotyrosyl protein phosphatase antibody
    • HAAP antibody
    • LMW-PTP antibody
    • LMW-PTPase antibody
    • Low molecular weight cytosolic acid phosphatase antibody
    • Low molecular weight phosphotyrosine protein phosphatase antibody
    • PAP1 antibody
    • PAP2 antibody
    • PPAC_HUMAN antibody
    • Protein tyrosine phosphatase antibody
    • PTPase antibody
    • Purple acid phosphatase antibody
    • Red cell acid phosphatase 1 antibody
    see all

Anti-Acid Phosphatase antibody 图像

  • Immunohistochemical analysis of Mytilus galloprovincialis digestive gland tissue after exposure to pollutant, staining Acid Phosphatase with ab2108.

    Tissue was fixed in 4% formaldehyde and embedded in paraffin. Sections were blocked with 1% BSA at room temperature for 1 hour before incubating with primary antibody (1/2500) overnight at 4°C. An anti-rabbit peroxidase-conjugated IgG was used as the secondary antibody. Staining was detected using AEC.

Anti-Acid Phosphatase antibody (ab2108)参考文献

This product has been referenced in:
  • Izagirre U  et al. Time-course study of the early lysosomal responses to pollutants in mussel digestive cells using acid phosphatase as lysosomal marker enzyme. Comp Biochem Physiol C Toxicol Pharmacol 149:587-97 (2009). IHC-P ; Other . Read more (PubMed: 19174192) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. The supplier used purified enzyme as a positive control in the Westerns for this antibody. I'm afraid I don't know if potato homogenate would work. If you have any more questions, please contact us again.

96kDa, non-reducing.

This antibody can be directly conjugated with a fluorescent label or used in conjunction with a fluorescently labelled secondary antibody in the applications listed on the datasheet.

I have made enquiries about the nature of Potato Acid Phosphatase used as the immunogen for this product, but I am afraid I have been unable to find any information regarding whether it is the particular type of acid phosphatase you have specified

This antibody has not yet been tested for use with paraffin embedded tissue.

Acid phosphatase is a heterodimer of 44 kDa units and the antibody will recognize EI, EII, and EIII forms of the enzyme. The immunogen is the enqyme from potato. We do not have the amino acid sequence of this enzyme.

This antibody has not, to our knowledge, been tested on rat tissue. We have no data on its use in IHC.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"