Anti-Acid Phosphatase 2抗体(ab54721)

概述

  • 产品名称Anti-Acid Phosphatase 2抗体
    参阅全部 Acid Phosphatase 2 一抗
  • 描述
    小鼠单克隆抗体to Acid Phosphatase 2
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human, Chimpanzee, Ferret, Chlamydia psittaci, Hepatitis B virus, Orangutan, Scorpion
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-424 of Human Acid Phosphatase 2

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2b
  • 轻链类型lambda
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-P
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 疾病相关Defects in ACP2 are a cause of acid phosphatase deficiency (ACPHD) [MIM:200950]. The clinical features are intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding, and death in early infancy. Lysosomal acid phosphatase is deficient in cultured fibroblasts and multiple tissues.
    • 序列相似性Belongs to the histidine acid phosphatase family.
    • 翻译后修饰The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.
      N-glycosylated. The intermediates formed during enzymatic deglycosylation suggest that all eight predicted N-glycosylation sites are used.
    • 细胞定位Lysosome membrane. Lysosome lumen. The soluble form arises by proteolytic processing of the membrane-bound form.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acid phosphatase 2 lysosomal antibody
      • ACP 2 antibody
      • ACP2 antibody
      • LAP antibody
      • Lysosomal acid phosphatase 2 antibody
      • Lysosomal acid phosphatase antibody
      • PPAL_HUMAN antibody
      see all

    Anti-Acid Phosphatase 2 antibody 图像

    • Acid Phosphatase 2 antibody (ab54721) used in immunofluorescence at 10ug/ml on HeLa cells.
    • Acid Phosphatase 2 antibody (ab54721) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.
    • Western blot against tagged recombinant protein immunogen using ab54721 Acid Phosphatase 2 antibody at 1ug/ml. Predicted band size of immunogen is 72 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • ab54721 at 10 ug/ml staining Acid Phosphatase 2 in human Hela cells by Immunocytochemistry / Immunofluorescence.

    Anti-Acid Phosphatase 2 antibody (ab54721)参考文献

    ab54721 has not yet been referenced specifically in any publications.

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