使用敲除细胞株进行验证

Anti-ACF1 / BAZ1A抗体(ab56843)

概述

  • 产品名称Anti-ACF1 / BAZ1A抗体
    参阅全部 ACF1 / BAZ1A 一抗
  • 描述
    小鼠单克隆抗体to ACF1 / BAZ1A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: LVSKIQVPDY YDIIKKPIAL NIIREKVNKC EYKLASEFID DIELMFSNCF EYNPRNTSEA KAGTRLQAFF HIQAQKLGLH VTPSNVDQVS TPPAAKKSRI , corresponding to amino acids 1457-1557 of Human ACF1/ BAZ1A

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab56843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
      Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
    • 组织特异性Highly expressed in testis and at low or undetectable levels in other tissues analyzed.
    • 序列相似性Belongs to the WAL family.
      Contains 1 bromo domain.
      Contains 1 DDT domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 WAC domain.
    • 细胞定位Nucleus. May target the CHRAC complex to heterochromatin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acf1 antibody
      • ACF1, drosophila, homolog of antibody
      • ATP dependent chromatin remodelling protein antibody
      • ATP utilizing chromatin assembly and remodeling factor 1 antibody
      • ATP-dependent chromatin-remodeling protein antibody
      • ATP-utilizing chromatin assembly and remodeling factor 1 antibody
      • B930060C03 antibody
      • Baz1a antibody
      • BAZ1A_HUMAN antibody
      • BC065123 antibody
      • Bromodomain adjacent to zinc finger domain 1A antibody
      • Bromodomain adjacent to zinc finger domain protein 1A antibody
      • cbp146 antibody
      • CHRAC subunit ACF1 antibody
      • DKFZp586E0518 antibody
      • FLJ14383 antibody
      • Gtl5 antibody
      • hACF1 antibody
      • hWALp1 antibody
      • WALp1 antibody
      • WCRF180 antibody
      • Williams syndrome transcription factor related chromatin remodeling factor 180 antibody
      • Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody
      see all

    Anti-ACF1 / BAZ1A antibody 图像

    • Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: BAZ1A knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab56843 observed at 170 kDa. Red - loading control, ab176560, observed at 52 kDa.

      ab56843 was shown to recognize ACF1 / BAZ1A when ACF1 / BAZ1A knockout samples were used, along with additional cross-reactive bands. Wild-type and ACF1 / BAZ1A knockout samples were subjected to SDS-PAGE. Ab56843 and ab176560 (loading control to GAPDH) were diluted at 1 µg/ml  and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot against tagged recombinant protein immunogen using ab56843 ACF1 / BAZ1A antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-ACF1 / BAZ1A antibody (ab56843)参考文献

    ab56843 has not yet been referenced specifically in any publications.

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