Anti-ACF1 / BAZ1A抗体(ab22233)

概述

  • 产品名称Anti-ACF1 / BAZ1A抗体
    参阅全部 ACF1 / BAZ1A 一抗
  • 描述
    小鼠多克隆抗体to ACF1 / BAZ1A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein:

    PTALPPSEPKVDQKLPRSSERRGSGGGTQFPARSRAVAAGEAAARGAAGP ERGSPLGRRVSPRCLCSGEGGQVAVGVIAGKRGRRGRDGSRRAPGGREMP

    , corresponding to amino acids 53/152 of Human BAZ1A

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 178 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
    Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
  • 组织特异性Highly expressed in testis and at low or undetectable levels in other tissues analyzed.
  • 序列相似性Belongs to the WAL family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 WAC domain.
  • 细胞定位Nucleus. May target the CHRAC complex to heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acf1 antibody
    • ACF1, drosophila, homolog of antibody
    • ATP dependent chromatin remodelling protein antibody
    • ATP utilizing chromatin assembly and remodeling factor 1 antibody
    • ATP-dependent chromatin-remodeling protein antibody
    • ATP-utilizing chromatin assembly and remodeling factor 1 antibody
    • B930060C03 antibody
    • Baz1a antibody
    • BAZ1A_HUMAN antibody
    • BC065123 antibody
    • Bromodomain adjacent to zinc finger domain 1A antibody
    • Bromodomain adjacent to zinc finger domain protein 1A antibody
    • cbp146 antibody
    • CHRAC subunit ACF1 antibody
    • DKFZp586E0518 antibody
    • FLJ14383 antibody
    • Gtl5 antibody
    • hACF1 antibody
    • hWALp1 antibody
    • WALp1 antibody
    • WCRF180 antibody
    • Williams syndrome transcription factor related chromatin remodeling factor 180 antibody
    • Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody
    see all

Anti-ACF1 / BAZ1A antibody 图像

  • All lanes : Anti-ACF1 / BAZ1A antibody (ab22233) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 178 kDa

Anti-ACF1 / BAZ1A antibody (ab22233)参考文献

ab22233 has not yet been referenced specifically in any publications.

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