概述

  • 产品名称
    Anti-Acetylcholinesterase抗体[ZR3]
    参阅全部 Acetylcholinesterase 一抗
  • 描述
    小鼠单克隆抗体[ZR3] to Acetylcholinesterase
  • 特异性
    This antibody reacts with both the soluble and membrane-associated AChE.
  • 经测试应用
    适用于: Inhibition Assay, IHC-Fr, IP, ICC/IFmore details
    不适用于: WB
  • 种属反应性
    与反应: Rat, Rabbit, Guinea pig, Cat, Human
  • 免疫原

    Other Immunogen Type corresponding to Rat Acetylcholinesterase. Purified rat brain acetylcholinesterase.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    ZR3
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
EMSA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Immunohistochemical staining of Ach in rat brain results in staining of nerve fibers and terminals.

IP 1/1000.
ICC/IF Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
    • 组织特异性
      Isoform H is highly expressed in erythrocytes.
    • 序列相似性
      Belongs to the type-B carboxylesterase/lipase family.
    • 细胞定位
      Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ACEE antibody
      • ACES_HUMAN antibody
      • Acetylcholinesterase antibody
      • AChE antibody
      • Apoptosis related acetylcholinesterase antibody
      • ARACHE antibody
      • N ACHE antibody
      • N-ACHE antibody
      • YT antibody
      • YT blood group antibody
      see all

    Anti-Acetylcholinesterase antibody [ZR3] 图像

    • Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) ab2802 shows staining in C6 glioma cells. Acetylcholinesterase staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase ab2802 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) ab2802 shows staining in U251 glioma cells. Acetylcholinesterase staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase ab2802 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    Anti-Acetylcholinesterase antibody [ZR3] (ab2802)参考文献

    This product has been referenced in:
    • Rakonczay Z & Brimijoin S Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species. J Neurochem 46:280-7 (1986). Read more (PubMed: 3510009) »

    See 1 Publication for this product

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