Anti-Acetylated alpha Tubulin抗体[6-11B-1] (ab24610)

概述

  • 产品名称Anti-Acetylated alpha Tubulin抗体[6-11B-1]
  • 描述
    小鼠单克隆抗体[6-11B-1] to Acetylated alpha Tubulin
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human, Monkey, Sea Urchin
    预测可用于: Cow
  • 免疫原

    Tissue, cells or virus corresponding to Acetylated alpha Tubulin.

  • 阳性对照
    • In Western Blot, this antibody gave a positive signal in mouse brain tissue lysate and in the following whole cell lysates: HeLa; NIH3T3; PC12.
  • 常规说明

    Production of this antibody has been changed on 8th April 2016. This antibody is now purified from tissue culture supernatant. This shouldn’t affect the use of this antibody but if you have any issues, please contact our Scientific Support team.

    This antibody binds to primary cilia, centrioles, mitotic spindles, midbodies and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • 纯度Tissue culture supernatant
  • Primary antibody说明This antibody binds to primary cilia, centrioles, mitotic spindles, midbodies and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells.
  • 克隆单克隆
  • 克隆编号6-11B-1
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µl for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.03 - 0.06 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta-tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. Acetylated alpha tubulin is present in various microtubule structures and plays a role in stabilizing the structures of all microtubules.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • alpha 1b antibody
    • Alpha-tubulin ubiquitous antibody
    • K-ALPHA-1 antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin K-alpha-1 antibody
    see all

Anti-Acetylated alpha Tubulin antibody [6-11B-1] 图像

  • All lanes : Anti-Acetylated alpha Tubulin antibody [6-11B-1] (ab24610) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 140 kDa,25 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
  • ab24610 staining Acetylated alpha Tubulin in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3% PFA + 0.1% GA and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA + 0.5% Triton X-100) for 1 hour at 21°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody.

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  • ab24610 at 1/100 dilution staining acetylated alpha tubulinin in prostate carcinoma by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, permeabilized in Triton X-100 prior to blocking in 1% serum for 1 hour at 27°C and then incubated with ab24610 for 12 hours at 4°C. Alexa Fluor® 546 donkey polyclonal to mouse Ig, diluted 1/500, was used as the secondary antibody.

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  • ICC/IF image of ab24610 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab24610, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT™ FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ICC/IF image of ab24610 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24610, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab24610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24610, 1µg/1x106 cells) for 30 min at 22ºC. (This data was generated from a purified version of the antibody. Some lots are produced as ascites fluid. We suggest 1µl/1x106 cells for ascites preparations). The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Acetylated alpha Tubulin antibody [6-11B-1] (ab24610)参考文献

This product has been referenced in:
  • Courtheoux T  et al. Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase. Nat Commun 7:12810 (2016). ICC/IF ; Human . Read more (PubMed: 27641145) »
  • Stoetzel C  et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun 7:13586 (2016). ICC/IF ; Human . Read more (PubMed: 27882921) »

See all 51 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fibroblasts)
Permeabilization Yes - PBS1X, 0.2% triton
Specification Fibroblasts
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 29 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step 3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Monkey Cell (Kidney)
Specification Kidney
Permeabilization No
Fixative 3% PFA and 0.1% GA
Username

Dr. Aaron Halpen

Verified customer

提交于 Sep 29 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cultured Cells (Mouse Glioblastoma (GL261))
Permeabilization Yes - 0.3% TritonX-100 in 1X TBS
Specification Mouse Glioblastoma (GL261)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 28°C
Fixative Paraformaldehyde
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提交于 Sep 16 2014

Application Western blot
Loading amount 10000 cells
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Mouse Glioblastoma (GL261))
Specification Mouse Glioblastoma (GL261)
Treatment HDAC inhibitors for 24hrs
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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提交于 Sep 12 2014

Application Immunoprecipitation
Immuno-precipitation step Other - Protein G Dynabeads
Sample Human Cell lysate - other (H23)
Specification H23
Total protein in input 250 µg
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提交于 Dec 18 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (U87 human primary Glioblastoma)
Specification U87 human primary Glioblastoma
Permeabilization Yes - 0.3% triton X 100
Fixative Paraformaldehyde
Username

Abcam user community

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提交于 Nov 11 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neuro2A, mouse neuroblastoma)
Specification Neuro2A, mouse neuroblastoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Nov 11 2013

The FACS data on the datasheet was from cells stained with a purified version of the antibody for which the concentration was known to be 0.4 mg/ml. The antibody is currently produced as ascites fluid. The amount of specific antibody in ascites is typi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Sheep Tissue sections (Sheep Kidney)
Specification Sheep Kidney
Permeabilization Yes - PBS Tween
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Gliobastoma cell line)
Specification Gliobastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Mr. Wenson David Rajan

Verified customer

提交于 May 10 2013

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