Anti-acetyl Lysine抗体(HRP) (ab23364)

概述

  • 产品名称Anti-acetyl Lysine抗体(HRP)
    参阅全部 acetyl Lysine 一抗
  • 描述
    兔多克隆抗体to acetyl Lysine (HRP)
  • 偶联物HRP
  • 特异性This antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Acetylated KLH conjugates.

  • 常规说明


    The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 0.1M PBS, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
Microarray: Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 3 kDa.

靶标

  • 相关性In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • 别名
    • pan acetyl Lysine antibody

Anti-acetyl Lysine antibody (HRP) (ab23364)参考文献

This product has been referenced in:
  • van Gent R  et al. SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner. PLoS One 9:e98438 (2014). WB ; Human . Read more (PubMed: 24875183) »

See 1 Publication for this product

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Thank you for your enquiry. I am sorry to hear that you have been having problems with ab23364 anti-acetyl Lysine. Quality is important to Abcam, if an antibody does not work as specified on the datasheet, we will offer a replacement or reimburseme...

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