Anti-acetyl Lysine抗体(Biotin) (ab26332)

概述

  • 产品名称Anti-acetyl Lysine抗体(Biotin)
    参阅全部 acetyl Lysine 一抗
  • 描述
    兔多克隆抗体to acetyl Lysine (Biotin)
  • 偶联物Biotin
  • 特异性ab26332 detects proteins containing acetylated lysine residues. The product has been cross-adsorbed against the non-acetylated lysine to remove antibodies that may detect the non-acetylated form of the amino acid.
  • 经测试应用适用于: WB, IP, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Acetylated KLH.

  • 常规说明Storage below -20°C is not recommended.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.1M PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was purified on an acetylated lysine bound immunoaffinity column.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab26332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
ELISA
  • 应用说明ELISA: Use at a concentration of 0.25 µg/ml.
    IF: 1/50.
    IP: Use 10µg per sample.
    WB: Use at a concentration of 1 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
    • 别名
      • pan acetyl Lysine antibody

    Anti-acetyl Lysine antibody (Biotin) (ab26332)参考文献

    ab26332 has not yet been referenced specifically in any publications.

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