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Synthetic acetylated peptide derived from Lysine-acetlyated proteins (G-A-KA-KA-KA-KA-KA-KA).
Our Abpromise guarantee covers the use of ab61257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 85 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of ab61257 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61257 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Image courtesy of an anonymous Abreview.293 histone (acid extracted) loaded at 2µg.Blocking step performed using 0.3% BSA for 12 hours at 4°C.ab61257 used at a 1/1000 dilution for 1 hour at 25°C.The secondary used was an HRP conjugated donkey anti-rabbit IgG polyclonal used at a 1/2000 dilution.
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