Anti-Acetyl Coenzyme A Carboxylase (phospho S79)抗体(ab31931)

概述

  • 产品名称Anti-Acetyl Coenzyme A Carboxylase (phospho S79)抗体
    参阅全部 Acetyl Coenzyme A Carboxylase 一抗
  • 描述
    兔多克隆抗体to Acetyl Coenzyme A Carboxylase (phospho S79)
  • 特异性This antibody recognizes Acetyl Coenzyme A Carboxylase phosphorylated at Ser79.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Sheep, Goat, Chicken, Cow
  • 免疫原

    Synthetic peptide: C-HMRSSM[pS]GLHLVK conjugated to KLH, corresponding to amino acids 73-85 of Rat Acetyl Coenzyme A Carboxylase.

  • 阳性对照
    • RIPA lysates from mouse heart cytosol.

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应用

Our Abpromise guarantee covers the use of ab31931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding.

靶标

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody 图像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 265 kDa

    MCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol (ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79) (ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 265 kDa

    HepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). Decreased expression of Acetyl Coenzyme A Carboxylase (phospho S79) (ab31931) in HepG2 cells correlates with an increase in KU-55933 concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)参考文献

ab31931 has not yet been referenced specifically in any publications.

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