Anti-Acetyl Coenzyme A Carboxylase (phospho S79)抗体(ab31931)
Key features and details
- Rabbit polyclonal to Acetyl Coenzyme A Carboxylase (phospho S79)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Acetyl Coenzyme A Carboxylase (phospho S79)抗体
参阅全部 Acetyl Coenzyme A Carboxylase 一抗 -
描述
兔多克隆抗体to Acetyl Coenzyme A Carboxylase (phospho S79) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Sheep, Goat, Chicken, Cow -
免疫原
Synthetic peptide corresponding to Rat Acetyl Coenzyme A Carboxylase aa 50-150 (phospho S79) conjugated to keyhole limpet haemocyanin.
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阳性对照
- RIPA lysates from mouse heart cytosol.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31931于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding. |
靶标
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功能
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
组织特异性
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
通路
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
疾病相关
Acetyl-CoA carboxylase 1 deficiency -
序列相似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
翻译后修饰
Phosphorylation on Ser-1263 is required for interaction with BRCA1. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 396504 Chicken
- Entrez Gene: 281590 Cow
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Omim: 200350 Human
- Omim: 601557 Human
- SwissProt: Q9TTS3 Cow
- SwissProt: O00763 Human
see all -
别名
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
图片
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MCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol (ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79) (ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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HepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). Decreased expression of Acetyl Coenzyme A Carboxylase (phospho S79) (ab31931) in HepG2 cells correlates with an increase in KU-55933 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab31931 被引用在 2 文献中.
- Chen XY et al. LB100 ameliorates nonalcoholic fatty liver disease via the AMPK/Sirt1 pathway. World J Gastroenterol 25:6607-6618 (2019). PubMed: 31832001
- Calcutt NA et al. Selective antagonism of muscarinic receptors is neuroprotective in peripheral neuropathy. J Clin Invest 127:608-622 (2017). PubMed: 28094765