Anti-Acetyl Coenzyme A carboxylase alpha抗体(ab72046)

概述

  • 产品名称Anti-Acetyl Coenzyme A carboxylase alpha抗体
    参阅全部 Acetyl Coenzyme A carboxylase alpha 一抗
  • 描述
    兔多克隆抗体to Acetyl Coenzyme A carboxylase alpha
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Cow, Human
    预测可用于: Rat, Sheep, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 2333 and the C-terminus (residue 2383) of human acetyl Coenzyme A carboxylase alpha (NP_942131.1).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

应用

Our Abpromise guarantee covers the use of ab72046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    Anti-Acetyl Coenzyme A carboxylase alpha antibody 图像

    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 266 kDa
      Observed band size : 266 kDa
      Additional bands at : 130 kDa,90 kDa. We are unsure as to the identity of these extra bands.
    • IHC image of ab72046 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72046, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Sheep Tissue lysate - whole (Liver)
    Gel Running Conditions Reduced Denaturing (4-20% Tris Glycine Gels)
    Loading amount 75 µg
    Specification Liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. John Odhiambo

    Verified customer

    提交于 Oct 14 2015

    Application Western blot
    Sample Pig Tissue lysate - other (Muscle)
    Loading amount 8 µg
    Specification Muscle
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"