Anti-Acetyl CoA synthetase抗体(ab66038)


  • 产品名称Anti-Acetyl CoA synthetase抗体
    参阅全部 Acetyl CoA synthetase 一抗
  • 描述
    兔多克隆抗体to Acetyl CoA synthetase
  • 经测试应用适用于: WB, ICC/IF, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Acetyl CoA synthetase.

    (Peptide available as ab71894.)

  • 阳性对照
    • This antibody gave a positive signal in Human Liver and Human Colon Tissue lysates and in the following whole cell lysates: HepG2; U-87 MG; Caco 2.




Our Abpromise guarantee covers the use of ab66038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.


  • 功能Activates acetate so that it can be used for lipid synthesis or for energy generation.
  • 序列相似性Belongs to the ATP-dependent AMP-binding enzyme family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACAS2 antibody
    • AceCS antibody
    • Acetate CoA ligase antibody
    • Acetate thiokinase antibody
    • Acetate--CoA ligase antibody
    • Acetyl CoA synthetase antibody
    • Acetyl Coenzyme A synthetase 2 (ADP forming) antibody
    • Acetyl coenzyme A synthetase cytoplasmic antibody
    • Acetyl-CoA synthetase antibody
    • Acetyl-coenzyme A synthetase antibody
    • ACS antibody
    • ACSA antibody
    • ACSA_HUMAN antibody
    • ACSS2 antibody
    • Acyl activating enzyme antibody
    • Acyl CoA synthetase short chain family member 2 antibody
    • Acyl-activating enzyme antibody
    • Acyl-CoA synthetase short-chain family member 2 antibody
    • Cytoplasmic acetyl coenzyme A synthetase antibody
    • cytoplasmic antibody
    • MYH7B antibody
    see all

Anti-Acetyl CoA synthetase antibody 图像

  • All lanes : Anti-Acetyl CoA synthetase antibody (ab66038) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : Human colon tissue lysate - total protein (ab30051)
    Lane 5 : Caco 2 whole cell lysate (ab3950)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
    Additional bands at : 100 kDa,38 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Acetyl CoA synthetase was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to Acetyl CoA synthetase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab66038.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 79kDa: Acetyl CoA synthetase.
  • IHC image of Acetyl CoA synthetase staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66038, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab66038 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66038, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.

Anti-Acetyl CoA synthetase antibody (ab66038)参考文献

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Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Other - Trueblot from rockland
Sample Human Cell lysate - whole cell (human fibroblast)
Specification human fibroblast
Total protein in input 500 µg

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Verified customer

提交于 Aug 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (human fibroblast)
Specification human fibroblast
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jun 10 2013