Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human hepatoma HepG2)
Specification
human hepatoma HepG2
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/150
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2.5% BSA 2% goat serum PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Natasha Snider
Verified customer
提交于 Mar 16 2011