概述

  • 产品名称Anti-ACAT2抗体
    参阅全部 ACAT2 一抗
  • 描述
    兔多克隆抗体to ACAT2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human ACAT2.

    (Peptide available as ab91442.)

  • 阳性对照
    • This antibody gave a positive signal in human liver tissue lysate as well as the following whole cell lysates: HepG2; HEK293; K562.

性能

应用

Our Abpromise guarantee covers the use of ab74901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

  • 序列相似性Belongs to the thiolase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acat2 antibody
    • acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetyl CoA acetyltransferase antibody
    • Acetyl CoA transferase like protein antibody
    • acetyl Coenzyme A acetyltransferase 2 antibody
    • Acetyl-CoA acetyltransferase antibody
    • Acetyl-CoA transferase-like protein antibody
    • Acyl Coenzyme A cholesterol acyltransferase 2 antibody
    • Cytosolic acetoacetyl-CoA thiolase antibody
    • cytosolic antibody
    • SOAT2 antibody
    • THIC_HUMAN antibody
    see all

Anti-ACAT2 antibody 图像

  • All lanes : Anti-ACAT2 antibody (ab74901) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 90 seconds
  • ICC/IF image of ab74901 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74901, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml.

Anti-ACAT2 antibody (ab74901)参考文献

ab74901 has not yet been referenced specifically in any publications.

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