Anti-ACAT1抗体(ab104284)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ACAT1
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (GHQDVMVAGG MESMSNVPYV MNRGSTPYGG VKLEDLIVKD GLTDVYNKIH) of Human ACAT1 (NP_000010).

  • 阳性对照
    • HepG2 cell lysate

应用

Our Abpromise guarantee covers the use of ab104284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Plays a major role in ketone body metabolism.
  • 疾病相关
    Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • 序列相似性
    Belongs to the thiolase family.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACAT 1 antibody
    • ACAT antibody
    • acat1 antibody
    • Acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetoacetyl-CoA thiolase antibody
    • Acetyl CoA acetyltransferase, mitochondrial antibody
    • Acetyl Coenzyme A acetyltransferase 1 antibody
    • Acetyl-CoA acetyltransferase antibody
    • acetyl-coa acetyltransferase precursor, mitochondrial antibody
    • Acetyl-CoA thiolase, mitochondrial antibody
    • acetyl-Coenzyme A acetyltransferase 1 antibody
    • MAT antibody
    • mitochondrial acetoacetyl-CoA thiolase antibody
    • mitochondrial antibody
    • RATACAL antibody
    • T2 antibody
    • testicular tissue protein Li 198 antibody
    • THIL antibody
    • THIL_HUMAN antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal heart tissue labelling ACAT1 with ab104284.

  • Anti-ACAT1 antibody (ab104284) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 41 kDa
  • All lanes : Anti-ACAT1 antibody (ab104284) at 1/2000 dilution

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Mouse embryonic fibroblast whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Mr Dongil Kim

    See Abreview

文献

ab104284 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2, 293T, MEF)
Loading amount
20 µg
Specification
HepG2, 293T, MEF
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7%
Username

Mr. Dongil Kim

Verified customer

提交于 Jan 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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