Anti-ACAT1抗体[9H10AB4] (ab110290)
Key features and details
- Mouse monoclonal [9H10AB4] to ACAT1
- Suitable for: ICC, Flow Cyt, IP, IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-ACAT1抗体[9H10AB4]
参阅全部 ACAT1 一抗 -
描述
小鼠单克隆抗体[9H10AB4] to ACAT1 -
宿主
Mouse -
经测试应用
适用于: ICC, Flow Cyt, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- ICC: Human HDFn cells IHC-P: Human cerebellumIP: HepG2 cells and Human liver mitochondria Flow Cyt: HeLa cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
9H10AB4 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/100. Perform heat mediated antigen retrieval via the pressure cooker method (1 minute, with 1 mmol EDTA at pH8) before commencing with IHC staining protocol.
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说明 |
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ICC
Use a concentration of 5 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method (1 minute, with 1 mmol EDTA at pH8) before commencing with IHC staining protocol. |
靶标
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功能
Plays a major role in ketone body metabolism. -
疾病相关
Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. -
序列相似性
Belongs to the thiolase family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 38 Human
- Omim: 607809 Human
- SwissProt: P24752 Human
- Unigene: 232375 Human
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别名
- ACAT 1 antibody
- ACAT antibody
- acat1 antibody
see all
图片
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Immunocytochemistry image of ab110290 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (9H10AB4, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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ab110290 immunocaptured from HepG2 cells (lane 1) and Human liver mitochondria (lane 2)
Predicted molecular weight is 44 kDa. -
ab110290, at 1 µg/mL, staining ACAT1 in HeLa (blue) or in an isotype control antibody (red) and analyzed by flow cytometry.
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ACAT1 immunohistochemistry in human cerebellum visualized with ab110290. ACAT1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of ACAT1 immunoreactivity in the Purkinje cell bodies. The functional significance of this pattern is unknown at present but this antibody offers the opportunity to investigate it in more detail.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab110290 被引用在 1 文献中.
- Guan J et al. Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1. J Cell Mol Med 24:14039-14049 (2020). PubMed: 33103371