RabMAb

Anti-ACADM抗体[EPR3708] (ab92461)

概述

应用

Our Abpromise guarantee covers the use of ab92461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
IHC-P
ICC/IF
Flow Cyt
  • 应用说明ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.

    Is unsuitable for Flow Cyt.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能This enzyme is specific for acyl chain lengths of 4 to 16.
    • 通路Lipid metabolism; mitochondrial fatty acid beta-oxidation.
    • 疾病相关Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
    • 序列相似性Belongs to the acyl-CoA dehydrogenase family.
    • 细胞定位Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ACAD 1 antibody
      • ACAD1 antibody
      • Acadm antibody
      • ACADM_HUMAN antibody
      • Acyl coenzyme A dehydrogenase antibody
      • Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
      • FLJ18227 antibody
      • FLJ93013 antibody
      • FLJ99884 antibody
      • MCAD antibody
      • MCADH antibody
      • Medium chain acyl CoA dehydrogenase antibody
      • Medium chain fatty acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
      • Medium-chain specific acyl-CoA dehydrogenase antibody
      • mitochondrial antibody
      see all

    Anti-ACADM antibody [EPR3708] 图像

    • ab92461, at 1/100 dilution, staining ACADM in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry.
    • Immunofluorescent staining of ACADM in HeLa cells using ab92461 at 1/100 dilution.
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ACADM with unpurified ab92461 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • All lanes : Anti-ACADM antibody [EPR3708] (ab92461) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : fetal liver lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-Rabbit Ig at 1/2000 dilution

      Predicted band size : 47 kDa

    Anti-ACADM antibody [EPR3708] (ab92461)参考文献

    This product has been referenced in:
    • Mells JE  et al. Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol 302:G225-35 (2012). WB ; Mouse . Read more (PubMed: 22038829) »
    • Perrone CE  et al. Methionine restriction effects on mitochondrial biogenesis and aerobic capacity in white adipose tissue, liver, and skeletal muscle of F344 rats. Metabolism 59:1000-11 (2010). WB ; Rat . Read more (PubMed: 20045141) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Sample Mouse Tissue lysate - whole (Brain)
    Specification Brain
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 27 2014

    I appreciate the time that has been spent on these experiments and would be pleased to arrange, in compensation, a replacement of ab92461 with another anti-ACADM such as ab110296. Alternatively I can also arrange a credit note. I look forward to hea...

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    Thank you very much for your email. My colleague is in contact with the laboratory in order to try to find out what the problem might is. Indeed we have to investigate this issue further as the quality of our products is most important. We have had no ...

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    Thank you for sending the image. We can’t make assumptions for the specificity of an antibody because of its same size band specificity of as of beta actin. Many protein have similar sizes, so experimental evidence is always good to prove the cross...

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    Thank you for contacting us. The antibody is specific against ACADM protein only; ab92461 should not recognize the beta actin protein. Could you please explain why you think the antibody is detecting the beta actin protein? In regards to the exp...

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    Thank you for your enquiry regarding ab92461 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. I appreciate the time you have spent on these experimen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"