Anti-ABLIM3抗体(ab67721)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to ABLIM3
  • 经测试应用
    适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-544 of Human ABLIM3

性能

应用

Our Abpromise guarantee covers the use of ab67721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
  • 组织特异性
    Expressed predominantly in heart and brain.
  • 序列相似性
    Contains 1 HP (headpiece) domain.
    Contains 4 LIM zinc-binding domains.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • abLIM-3 antibody
    • Ablim3 antibody
    • ABLM3_HUMAN antibody
    • Actin binding LIM protein 3 antibody
    • Actin binding LIM protein family member 3 antibody
    • Actin-binding LIM protein 3 antibody
    • Actin-binding LIM protein family member 3 antibody
    • HMFN1661 antibody
    • KIAA0843 antibody
    see all

图片

  • All lanes : Anti-ABLIM3 antibody (ab67721) at 1/500 dilution

    Lane 1 : ABLIM3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

    Predicted band size : 78 kDa
  • ab67721 staining ABLIM3 in murine cerebellum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with acetone, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab67721 at a 1/200 dilution for 24 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-mouse polyclonal used at a 1/400 dilution.

    See Abreview

文献

ab67721 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Amar Sahay

Verified customer

提交于 Mar 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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