1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/500 - 1/1000.
Is unsuitable for Flow Cyt.
May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.
Belongs to the ABI family. Contains 1 SH3 domain. Contains 1 t-SNARE coiled-coil homology domain.
Is a substrate for ABL1.
Cytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
Abl interacting protein 1 (SH3 containing protein) antibody
Abl interactor 2 antibody
Abl interactor protein 2b antibody
Abl-binding protein 3 antibody
AIP 1 antibody
Arg binding protein 1 antibody
Arg protein tyrosine kinase binding protein antibody
Arg-binding protein 1 antibody
Western blot - Anti-ABI2 antibody [EPR3906] (ab108340)This image is courtesy of an anonymous Abreview
Western blot analysis of Human fibroblast cell lysates (15μg/lane) labelling ABI2 with ab108340 at 1:1000. A Dyomics® 647-conjugated Goat anti-rabbit IgG monoclonal (1:5000) was used as the secondary antibody.
Crawford LJ et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis3:e90 (2014).
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