Synthetic peptide derived from an internal sequence of human ABHD8.
HT-29 and LOVO cell extracts
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ABHD8 belongs to the AB hydrolase superfamily. It contains an alpha/beta hydrolase fold and secretory lipase domains.The alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The core of each enzyme is an alpha/beta-sheet (rather than a barrel), containing 8 strands connected by helices.
ABHD 8 antibody
Abhydrolase domain containing 8 antibody
Anti-ABHD8 antibody 图像
Western blot - ABHD8 antibody (ab74682)
All lanes :
Anti-ABHD8 antibody (ab74682) at 1/500 dilution
Lane 1 :
HT-29 cell extracts (5-30µg)
Lane 2 :
LOVO cell extracts (5-30µg)
Lane 3 :
HT-29 cell extracts (5-30µg), plus immunising peptide (5-10µg)
Predicted band size :
Observed band size :
45 kDa (
why is the actual band size different from the predicted?
Additional bands at :
60 kDa. We are unsure as to the identity of these extra bands.
Anti-ABHD8 antibody (ab74682)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"