IHC image of ABHD6 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87532, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - ABHD6 antibody (ab87532)
All lanes : Anti-ABHD6 antibody (ab87532) at 1/2000 dilution
Lane 1 : THP1 cell lysate at 60 µg Lane 2 : ECL cell lysate at 72 µg