Anti-ABHD6抗体(ab74681)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ABHD6
  • 特异性
    ab74681 detects endogenous levels of total ABHD6 protein.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human ABHD6.

  • 阳性对照
    • COLO cell extracts and A549 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab74681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-ABHD6 antibody 图像

  • ab74681 at 1/500-1/1000 dilution staining ABHD6 in A549 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-ABHD6 antibody (ab74681) at 1/500 dilution

    Lane 1 : COLO cell extracts
    Lane 2 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

Anti-ABHD6 antibody (ab74681)参考文献

ab74681 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (TSA cells)
Permeabilization
Yes - Triton
Specification
TSA cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Aug 03 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
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Abcam user community

Verified customer

提交于 Aug 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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