概述

  • 产品名称Anti-Abhd5抗体
    参阅全部 Abhd5 一抗
  • 描述
    兔多克隆抗体to Abhd5
  • 经测试应用适用于: IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Abhd5.

    (Peptide available as ab89382.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; A431; MOLT4; SAOS 2.

应用

Our Abpromise guarantee covers the use of ab73551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 25054327
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • 组织特异性Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • 疾病相关Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • 序列相似性Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • 发展阶段Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • 结构域The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 细胞定位Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
    • ABHD5 antibody
    • ABHD5_HUMAN antibody
    • Abhydrolase domain containing 5 antibody
    • Abhydrolase domain containing protein 5 antibody
    • Abhydrolase domain-containing protein 5 antibody
    • CDS antibody
    • CGI 58 antibody
    • CGI58 antibody
    • CGI58 protein antibody
    • IECN2 antibody
    • Lipid droplet-binding protein CGI-58 antibody
    • MGC8731 antibody
    • NCIE2 antibody
    see all

Anti-Abhd5 antibody 图像

  • All lanes : Anti-Abhd5 antibody (ab73551) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 4 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • ICC/IF image of ab73551 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73551, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Abhd5 antibody (ab73551)参考文献

This product has been referenced in:
  • Mason RR  et al. Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise. PLoS One 9:e103062 (2014). IHC-Fr ; Human . Read more (PubMed: 25054327) »

See 1 Publication for this product

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