概述

  • 产品名称Anti-Abhd5抗体
    参阅全部 Abhd5 一抗
  • 描述
    山羊多克隆抗体to Abhd5
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-FPERPDLADQDR

    , corresponding to internal sequence amino acids 181-192 of Human Abhd5.

  • 阳性对照
    • Human kidney and pancreas tissue; NIH3T3 lysate

应用

Our Abpromise guarantee covers the use of ab111984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 3.75 µg/ml.
ELISA 1/16000.

靶标

  • 功能Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • 组织特异性Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • 疾病相关Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • 序列相似性Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • 发展阶段Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • 结构域The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 细胞定位Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
    • ABHD5 antibody
    • ABHD5_HUMAN antibody
    • Abhydrolase domain containing 5 antibody
    • Abhydrolase domain containing protein 5 antibody
    • Abhydrolase domain-containing protein 5 antibody
    • CDS antibody
    • CGI 58 antibody
    • CGI58 antibody
    • CGI58 protein antibody
    • IECN2 antibody
    • Lipid droplet-binding protein CGI-58 antibody
    • MGC8731 antibody
    • NCIE2 antibody
    see all

Anti-Abhd5 antibody 图像

  • ab111984, at 3.75 µg/ml, staining Abhd5 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab111984, at 3.75 µg/ml, staining Abhd5 in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • Anti-Abhd5 antibody (ab111984) at 0.2 µg/ml + NIH3T3 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa

Anti-Abhd5 antibody (ab111984)参考文献

This product has been referenced in:
  • Gray NE  et al. Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes. J Biol Chem 287:8444-56 (2012). WB ; Mouse . Read more (PubMed: 22267746) »

See 1 Publication for this product

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