Anti-ABH2抗体(ab105340)

概述

  • 产品名称Anti-ABH2抗体
    参阅全部 ABH2 一抗
  • 描述
    兔多克隆抗体to ABH2
  • 特异性At least two isoforms are known to exist; ab105340 only recognizes the longer isoform. Predicted not to cross-react with other ALKBH members.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid synthetic peptide from near the C terminus of Human ABH2 (NP_001138847).

  • 阳性对照
    • Human kidney tissue lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab105340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with ABH2 peptide (ab139425).

靶标

  • 功能Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • 组织特异性Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • 序列相似性Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • 细胞定位Nucleus. Detected in replication foci during S-phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2OG Fe(II) oxy DC1 antibody
    • 9530023G02 antibody
    • ABH 2 antibody
    • ABH2 antibody
    • alkB alkylation repair homolog 2 antibody
    • ALKB2_HUMAN antibody
    • ALKBH2 alkB antibody
    • ALKBH2 antibody
    • Alkylated DNA repair protein alkB homolog antibody
    • Alkylated DNA repair protein alkB homolog 2 antibody
    • Alkylation repair homolog 2 antibody
    • Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody
    • AU016977 antibody
    • DNA oxidative demethylase ALKBH2 antibody
    • EC 1.14.11. antibody
    • FLJ99103 antibody
    • hABH2 antibody
    • mABH2 antibody
    • MGC90512 antibody
    • Oxy DC1 antibody
    see all

Anti-ABH2 antibody 图像

  • Lane 1 : Anti-ABH2 antibody (ab105340) at 1 µg/ml
    Lane 2 : Anti-ABH2 antibody (ab105340) at 2 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 29 kDa

Anti-ABH2 antibody (ab105340)参考文献

ab105340 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The blocking peptide for ab105340 has been added to our catalog as ab139425. I hope this helps, please let me know if you need any further assistance.

Thank you very much for your reply. My colleague is out of the office today but I'll be happy to help you with this.

We don't currently have blocking peptides available for ab105340 or ab56540, but I'm in the process of adding the peptide for...

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Thank you for your reply.

I hope that the positive control lysate will give clearer results for you. Based on your protocol, the only suggestion I would make would be to boil your samples for 5 minutes to ensure they are fully reduced and den...

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Thank you for your reply. It would be very helpful if you could please provide the below information about your protocol:

1) What type of samples are you testing? What species are they from, what lysis buffer was used, were they prepared with...

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Thank you for your reply.

As a positive control for both ab56540 and ab105340 I would recommend K-562 cell lysate (ab7911). Since both proteins are very widely expressed, I am not able to recommend a sample that would work as a negative contr...

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Thank you for your reply. The publications that are known to reference an Abcam product can be found on our online datasheets on the specific references tab. Below are PubMed IDs for the antibodies you are interested in.

MGMT (ab39253) - 2175...

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Thank you for contacting us.

The final dilutions should be optimized for your particular samples and protocols, but the following would be my recommended starting dilutions for WB with an overnight incubation:

MGMT (ab39253) - 1:500...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"