概述

  • 产品名称Anti-ABCF2抗体
    参阅全部 ABCF2 一抗
  • 描述
    兔多克隆抗体to ABCF2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of human ABCF2 using the numbering given in entry NP_009120.1.

  • 阳性对照
    • HeLa whole cell lysate 293T whole cell lysate NIH3T3 whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 71 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-ABCF2 antibody 图像

    • All lanes : Anti-ABCF2 antibody (ab86178) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Observed band size : 71 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds
    • Detection of Human ABCF2 by Immunoprecipitation, using ab86178 at 3µg/mg lysate. Image shows immunoprecipitated ABCF2 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with ab86178 at 1 µg/ml in lane 1, antibodies recognising a downstream eptiope in lanes 2 and 3, and control IgG in lane 4. Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-ABCF2 antibody (ab86178)参考文献

    ab86178 has not yet been referenced specifically in any publications.

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