Anti-ABCF1抗体[ABC5H06] (ab50976)

概述

  • 产品名称Anti-ABCF1抗体[ABC5H06]
    参阅全部 ABCF1 一抗
  • 描述
    小鼠单克隆抗体[ABC5H06] to ABCF1
  • 经测试应用适用于: WB, IP, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human ABCF1 fragment

性能

应用

Our Abpromise guarantee covers the use of ab50976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa).
IP Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

靶标

  • 功能Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
    Contains 2 ABC transporter domains.
  • 翻译后修饰Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes.
  • 细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC27 antibody
    • ABC50 antibody
    • ABCF1 antibody
    • ABCF1_HUMAN antibody
    • ATP binding cassette 50 (TNF alpha stimulated) antibody
    • ATP binding cassette 50 antibody
    • ATP binding cassette sub family F member 1 antibody
    • ATP binding cassette, sub family F (GCN20), member 1 antibody
    • ATP-binding cassette 50 antibody
    • ATP-binding cassette sub-family F member 1 antibody
    • AU041969 antibody
    • D17Wsu166e antibody
    • EST123147 antibody
    • GCN20 antibody
    • TNF alpha stimulated ABC protein antibody
    • TNF-alpha-stimulated ABC protein antibody
    • TNFalpha inducible ATP binding protein antibody
    • wu:fb79c06 antibody
    • wu:fc39a05 antibody
    • wu:fj94a08 antibody
    • zgc:85667 antibody
    see all

Anti-ABCF1 antibody [ABC5H06] 图像

  • Anti-ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution + RIPA lysate of HeLa cells at 500 µg

    Secondary
    Anti-mouse IgG at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 96 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-ABCF1 antibody [ABC5H06] (ab50976)参考文献

This product has been referenced in:
  • Pechenino AS  et al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB ; Rat . Read more (PubMed: 21750230) »

See 1 Publication for this product

Product Wall

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

Read More
Application Western blot
Sample Rat Tissue lysate - other (Heart--Left ventricle)
Loading amount 40 µg
Specification Heart--Left ventricle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Angela Pechenino

Verified customer

提交于 Feb 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"