• 产品名称Anti-ABCF1抗体
    参阅全部 ABCF1 一抗
  • 描述
    兔多克隆抗体to ABCF1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 150 and 200 of human ABCF1 (NP_001020262.1)

  • 阳性对照
    • HeLa and 293T whole cell lysate



Our Abpromise guarantee covers the use of ab86223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 96 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.


  • 功能Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
    Contains 2 ABC transporter domains.
  • 翻译后修饰Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes.
  • 细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC27 antibody
    • ABC50 antibody
    • ABCF1 antibody
    • ABCF1_HUMAN antibody
    • ATP binding cassette 50 (TNF alpha stimulated) antibody
    • ATP binding cassette 50 antibody
    • ATP binding cassette sub family F member 1 antibody
    • ATP binding cassette, sub family F (GCN20), member 1 antibody
    • ATP-binding cassette 50 antibody
    • ATP-binding cassette sub-family F member 1 antibody
    • AU041969 antibody
    • D17Wsu166e antibody
    • EST123147 antibody
    • GCN20 antibody
    • TNF alpha stimulated ABC protein antibody
    • TNF-alpha-stimulated ABC protein antibody
    • TNFalpha inducible ATP binding protein antibody
    • wu:fb79c06 antibody
    • wu:fc39a05 antibody
    • wu:fj94a08 antibody
    • zgc:85667 antibody
    see all

Anti-ABCF1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling ABCF1 with ab86223 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ABCF1 antibody (ab86223) at 0.025 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 96 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds
  • Detection of human ABCF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86223 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 1 second.

Anti-ABCF1 antibody (ab86223)参考文献

ab86223 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

Read More