概述

  • 产品名称Anti-ABCB5抗体
    参阅全部 ABCB5 一抗
  • 描述
    山羊多克隆抗体to ABCB5
  • 经测试应用适用于: Flow Cyt, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-QTQHRNTSKKAQ

    , corresponding to internal sequence amino acids 460-471 of Human ABCB5 (NP_848654.3).

  • 阳性对照
    • A431 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab77549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 20682318ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ELISA Use at an assay dependent concentration. antibody detection limit dilution 1:32000.
IHC-P Use at an assay dependent concentration. PubMed: 22865455

靶标

  • 功能Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas.
  • 组织特异性Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 2 ABC transporter domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABCB 5 antibody
    • ABCB5 antibody
    • ABCB5 P-gp antibody
    • ABCB5_HUMAN antibody
    • ABCB5alpha antibody
    • ABCB5beta antibody
    • ATP binding cassette protein antibody
    • ATP binding cassette sub family B (MDR/TAP) member 5 antibody
    • ATP binding cassette sub family B member 5 antibody
    • ATP-binding cassette sub-family B member 5 antibody
    • EST422562 antibody
    • P glycoprotein ABCB5 antibody
    • P-glycoprotein ABCB5 antibody
    see all

Anti-ABCB5 antibody 图像

  • Anti-ABCB5 antibody (ab77549) at 0.03 µg/ml + A431 cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 90 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Anti-ABCB5 antibody (ab77549) at 0.3 µg/ml + Human skin Lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa

Anti-ABCB5 antibody (ab77549)参考文献

This product has been referenced in:
  • Lai CY  et al. CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry. Cancer Res 72:5111-8 (2012). IHC-P ; Human . Read more (PubMed: 22865455) »
  • Cheung ST  et al. Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance. Gastroenterology 140:344-55 (2011). Flow Cyt ; Human . Read more (PubMed: 20682318) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that the immunogen for this antibody is located in the internal sequence amino acids 460-471 of Human ABCB5. The amino acids are intracellular according to SwissProt:

http://www.uniprot.org/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"