概述

  • 产品名称Anti-ABCA1抗体
    参阅全部 ABCA1 一抗
  • 描述
    兔多克隆抗体to ABCA1
  • 特异性ab7360 has previously been shown to work well in ICC/IF on HeLa cells. However recent batches of this antibody, do not work in this application. We would recommend ab18180 for researchers wanting to detect ABCA1 in ICC/IF. For further information, please contact our Scientific Support Team.
  • 经测试应用适用于: IP, ELISA, WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human ABCA1. Synthetic peptide: The immunogen is generated from within residues 1100-1300 of Human ABCA1
    Database link: O95477

应用

Our Abpromise guarantee covers the use of ab7360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 14660648
ELISA Use at an assay dependent concentration. PubMed: 21829447
WB 1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
IHC-P 1/500.
IHC-Fr 1/750. PubMed: 12547833

靶标

  • 功能cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • 组织特异性Widely expressed, but most abundant in macrophages.
  • 疾病相关Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • 结构域Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • 翻译后修饰Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC 1 antibody
    • ABC Transporter 1 antibody
    • ABC-1 antibody
    • ABC1 antibody
    • ABCA 1 antibody
    • ABCA1 antibody
    • ABCA1_HUMAN antibody
    • ATP binding Cassette 1 antibody
    • ATP binding cassette sub family A ABC1 member 1 antibody
    • ATP binding cassette sub family A member 1 antibody
    • ATP binding Cassette Transporter 1 antibody
    • ATP-binding cassette 1 antibody
    • ATP-binding cassette sub-family A member 1 antibody
    • ATP-binding cassette transporter 1 antibody
    • CERP antibody
    • Cholesterol efflux regulatory protein antibody
    • FLJ14958 antibody
    • HDLDT1 antibody
    • Membrane bound antibody
    • MGC164864 antibody
    • MGC165011 antibody
    • TD antibody
    • TGD antibody
    see all

Anti-ABCA1 antibody 图像



  • Predicted band size : 220 kDa

    The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.

    The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separ


  • Predicted band size : 220 kDa
    Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
    Lane 4: T09 uninduced lysate
    Lane 5: T09 induced lysate Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
    Lane 4: T09 uninduced lysate
    Lane 5: T09 induced lysate
  • All lanes : Anti-ABCA1 antibody (ab7360)

    Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
    Lane 2 : RAW 264.7 whole cell lysate untreated

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 220 kDa
  • ab7360, staining ABCA1 in prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-ABCA1 antibody (ab7360)参考文献

This product has been referenced in:
  • Wang XQ  et al. CLI-095 decreases atherosclerosis by modulating foam cell formation in apolipoprotein E-deficient mice. Mol Med Rep 14:49-56 (2016). Read more (PubMed: 27176130) »
  • Rappocciolo G  et al. Alterations in cholesterol metabolism restrict HIV-1 trans infection in nonprogressors. MBio 5:e01031-13 (2014). Flow Cyt ; Human . Read more (PubMed: 24781743) »

See all 26 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and pluripotent cells)
Permeabilization Yes - Triton X-100
Specification HeLa and pluripotent cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
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提交于 Apr 14 2016

Application Western blot
Sample Human Cell lysate - whole cell (SCC61 cells)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification SCC61 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Nov 13 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

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Thank you for your reply and all the additional information. This makes the problems you have been experiencing much clearer to understand. It is clear that you have spent a lot of time in trying to optimise these antibodies and I am sorry that they ar...

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I am very sorry that you have not been satisfied with the way we have handeled your complaints. I do understand and appreciate the amount of time you have spent it the laboratory. Our policy is that we are happy to o...

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Thank you for reporting the problems encountered with these antibodies and for taking the time to complete the questionnaire. I am sorry to hear that these antibodieshave not providing satisfactory results so far.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human astrocyte)
Loading amount 25 µg
Specification human astrocyte
Treatment treated with LXR ligand for 20 hrs
Gel Running Conditions Reduced Denaturing (4-15% gradient gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Aug 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293, THP1, mouse BMDM, mouse tissue)
Loading amount 15 µg
Specification HEK293, THP1, mouse BMDM, mouse tissue
Treatment Transfected with ABCA1 expression plasmid, Treat with 1uM TO-1317 for 24hr
Gel Running Conditions Reduced Denaturing
Blocking step 5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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提交于 Jan 26 2008

I'm sorry to hear you are having a problem with ab7360. I would like to suggest the following modifications to your protocol: The recommended dilution range for this antibody is 1/500 - 1/1000. You may be using it at too high a dilution. I di...

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Application Western blot
Sample Mouse Tissue lysate - whole (macrophage cell line)
Loading amount 15 µg
Specification macrophage cell line
Treatment 0.3mM dbcAMP 24 hr
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
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提交于 Sep 18 2006

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