概述

  • 产品名称Anti-ABAT抗体[EPR4433]
    参阅全部 ABAT 一抗
  • 描述
    兔单克隆抗体[EPR4433] to ABAT
  • 特异性Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human ABAT

  • 阳性对照
    • HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. antigen retrieval is recommended
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
  • 组织特异性Liver > pancreas > brain > kidney > heart > placenta.
  • 疾病相关Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
  • 序列相似性Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (S) 3 amino 2 methylpropionate transaminase antibody
    • (S)-3-amino-2-methylpropionate transaminase antibody
    • 4 aminobutyrate aminotransferase antibody
    • 4 aminobutyrate aminotransferase, mitochondrial antibody
    • 4-aminobutyrate aminotransferase antibody
    • ABAT antibody
    • FLJ17813 antibody
    • FLJ30272 antibody
    • GABA aminotransferase antibody
    • GABA AT antibody
    • GABA T antibody
    • GABA transaminase antibody
    • GABA transferase antibody
    • GABA-AT antibody
    • GABA-T antibody
    • GABAT antibody
    • GABT_HUMAN antibody
    • Gamma amino N butyrate transaminase antibody
    • Gamma-amino-N-butyrate transaminase antibody
    • hCG1984265 antibody
    • L AIBAT antibody
    • L-AIBAT antibody
    • LAIBAT antibody
    • mitochondrial antibody
    • NPD009 antibody
    see all

Anti-ABAT antibody [EPR4433] 图像

  • All lanes : Anti-ABAT antibody [EPR4433] (ab108249)

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Human fetal liver tissue lysate
    Lane 4 : rat brain tissue lysate
    Lane 5 : rat kidney tissue lysate
    Lane 6 : mouse brain tissue lysate


    Predicted band size : 56 kDa
  • ab108249, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.
  • Overlay histogram showing HepG2 cells stained with ab108249 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108249, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ABAT antibody [EPR4433] (ab108249)参考文献

This product has been referenced in:
  • De Ita-Pérez D  et al. Synchronization by food access modifies the daily variations in expression and activity of liver GABA transaminase. Biomed Res Int 2014:590581 (2014). WB ; Rat . Read more (PubMed: 24809054) »
  • Jo S  et al. GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease. Nat Med N/A:N/A (2014). Mouse . Read more (PubMed: 24973918) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (breast cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification breast cancer
Blocking step Milk as blocking agent for 1 hour(s) and 23 minute(s) · Concentration: 0.5% · Temperature: 25°C
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提交于 Dec 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"