Anti-beta 2 Defensin/BD-2抗体(ab9871)
Key features and details
- Goat polyclonal to beta 2 Defensin/BD-2
- Suitable for: Sandwich ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-beta 2 Defensin/BD-2抗体
参阅全部 beta 2 Defensin/BD-2 一抗 -
描述
山羊多克隆抗体to beta 2 Defensin/BD-2 -
宿主
Goat -
经测试应用
适用于: Sandwich ELISA, Neutralising, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human beta 2 Defensin/BD-2 aa 1 to the C-terminus. Highly pure (>98%) recombinant Human beta 2 Defensin/hBD-2. Full length mature beta 2 Defensin hBD-2 used as immunogen.
Database link: O15263 -
阳性对照
- recombinant beta 2 Defensin/BD-2 ab9872
-
常规说明
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Lyophilized:Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles. -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Constituent: PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
Primary antibody说明
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
ELISA pair antibody
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
|
Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
|
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 7 kDa.
To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
说明 |
---|
Sandwich ELISA
Use at an assay dependent concentration. To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 7 kDa. To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
靶标
-
功能
Has antibacterial activity. -
组织特异性
Expressed in the skin and respiratory tract. -
序列相似性
Belongs to the beta-defensin family. LAP/TAP subfamily. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 100289462 Human
- Entrez Gene: 1673 Human
- Omim: 602215 Human
- SwissProt: O15263 Human
- Unigene: 105924 Human
- Unigene: 721744 Human
-
别名
- BD 2 antibody
- BD-2 antibody
- BD2 antibody
see all
数据表及文件
-
Datasheet download
文献 (8)
ab9871 被引用在 8 文献中.
- Johnson V et al. Activated Mesenchymal Stromal Cell Therapy for Treatment of Multi-Drug Resistant Bacterial Infections in Dogs. Front Vet Sci 9:925701 (2022). PubMed: 35812842
- Chow L et al. Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells. Stem Cells Transl Med 9:235-249 (2020). PubMed: 31702119
- Smits JPH et al. Targeting the Cutaneous Microbiota in Atopic Dermatitis by Coal Tar via AHR-Dependent Induction of Antimicrobial Peptides. J Invest Dermatol 140:415-424.e10 (2020). PubMed: 31344386
- Landman S et al. Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model. Sci Rep 8:10044 (2018). PubMed: 29968819
- de Koning HD et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol 130:2611-20 (2010). PubMed: 20631729
- Pignatti P et al. Tracheostomy and related host-pathogen interaction are associated with airway inflammation as characterized by tracheal aspirate analysis. Respir Med 103:201-8 (2009). PubMed: 18980836
- Herr C et al. Suppression of pulmonary innate host defence in smokers. Thorax 64:144-9 (2009). PubMed: 18852155
- Fahlgren A et al. Increased expression of antimicrobial peptides and lysozyme in colonic epithelial cells of patients with ulcerative colitis. Clin Exp Immunol 131:90-101 (2003). PubMed: 12519391