驴抗兔IgG H&L (DyLight® 488)预吸附二抗(ab96919)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗兔IgG H&L (DyLight® 488)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cytmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
偶联物
DyLight® 488. Ex: 493nm, Em: 518nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96919于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/20000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/50 - 1/500.
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ICC/IF | (1) |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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说明 |
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WB
1/1000 - 1/20000. Predicted molecular weight: 36 kDa. |
IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
图片
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rabbit anti-actin antibody at 1 µg/ml + HeLa whole cell lysate at 30 µg
Secondary
Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919) at 1/1000 dilution (Donkey anti-rabbit IgG heavy and light chain cross-adsorbed antibody)
Predicted band size: 36 kDa -
Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919) was used as a secondary antibdoy at a dilution of 1/50.
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E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.
This image is courtesy of an anonymous Abreview
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (20)
ab96919 被引用在 20 文献中.
- Otte K et al. Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide. Biomedicines 10:N/A (2022). PubMed: 36140245
- Chen X et al. Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems. Neuron 110:2242-2257.e6 (2022). PubMed: 35643078
- Bohne P et al. Deletion of the P/Q-type calcium channel from serotonergic neurons drives male aggression in mice. J Neurosci 42:6637-53 (2022). PubMed: 35853721
- Matthews AT et al. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. Int J Mol Sci 22:N/A (2021). PubMed: 34299212
- Li Q et al. M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis. Front Immunol 12:707784 (2021). PubMed: 34354711