山羊抗小鼠IgG H&L (DyLight® 650) (ab96874)
Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
概述
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产品名称
山羊抗小鼠IgG H&L (DyLight® 650)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
经测试应用
适用于: ICC/IF, IHC-P, Flow Cytmore details -
免疫原
Other Immunogen Type corresponding to Mouse IgG.
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偶联物
DyLight® 650. Ex: 654nm, Em: 673nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 650 replaces DyLight® 649. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96874于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50 - 1/500.
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|
IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
Flow Cyt |
1/50 - 1/200.
|
说明 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
Flow Cyt
1/50 - 1/200. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab96874 被引用在 5 文献中.
- Zhang L et al. C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/ß-catenin and ERK signaling in streptozocin-induced diabetic nephropathy. FASEB J 33:6551-6563 (2019). PubMed: 30794428
- Kutchy NA et al. Sperm cellular and nuclear dynamics associated with bull fertility. Anim Reprod Sci 211:106203 (2019). PubMed: 31785643
- Cox D & Ecroyd H The small heat shock proteins aB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of a-synuclein. Cell Stress Chaperones 22:589-600 (2017). PubMed: 28337642
- Zhang L et al. C-reactive protein directly suppresses Th1 cell differentiation and alleviates experimental autoimmune encephalomyelitis. J Immunol 194:5243-52 (2015). PubMed: 25917100
- Li HY et al. Topological localization of monomeric C-reactive protein determines proinflammatory endothelial cell responses. J Biol Chem 289:14283-90 (2014). PubMed: 24711458