Anti-SMC3抗体(ab9263)
Key features and details
- Rabbit polyclonal to SMC3
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SMC3抗体
参阅全部 SMC3 一抗 -
描述
兔多克隆抗体to SMC3 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to Human SMC3 conjugated to keyhole limpet haemocyanin. Which represents a portion within the last 100 amino acids GenBank PID 4885399.
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阳性对照
- WB: HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH/3T3 whole cell lysate; IHC-P: Human breast carcinoma and mouse renal cell carcinoma; IP: Lysate from HEK293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
应用 | Ab评论 | 说明 |
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IP | (1) |
Use at 2-10 µg/mg of lysate.
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WB | (3) |
1/10000 - 1/25000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa).
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/10000 - 1/25000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. -
疾病相关
Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation. -
序列相似性
Belongs to the SMC family. SMC3 subfamily. -
结构域
The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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数据库链接
- Entrez Gene: 395188 Chicken
- Entrez Gene: 281729 Cow
- Entrez Gene: 9126 Human
- Entrez Gene: 13006 Mouse
- Entrez Gene: 29486 Rat
- Omim: 606062 Human
- SwissProt: O97594 Cow
- SwissProt: Q9UQE7 Human
see all -
别名
- BAM antibody
- Bamacan antibody
- Basement membrane associated chondroitin proteoglycan antibody
see all
图片
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All lanes : Anti-SMC3 antibody (ab9263) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : TCMK-1 (mouse epithelial cell line from kidney transformed with SV40 viral antigen) whole cell lysate
Lane 8 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 141.5 kDa
Exposure time: 3 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
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Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: ab9263 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated SMC3, ab9263 was used at 0.04 µg/mL.
Detection: Chemiluminescence with an exposure time of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (84)
ab9263 被引用在 84 文献中.
- Morgan SM et al. The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity. Nat Commun 13:187 (2022). PubMed: 35039491
- Zhang K et al. The TERB1 MYB domain suppresses telomere erosion in meiotic prophase I. Cell Rep 38:110289 (2022). PubMed: 35081355
- Gines MM & Wendt KS A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing. Methods Mol Biol 2458:113-122 (2022). PubMed: 35103965
- Przanowska RK et al. Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors. Cell Rep 38:110361 (2022). PubMed: 35172143
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136