Anti-Nanog抗体(ab80892)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: ICC, ICC/IF, IHC-P, Flow Cyt, IHC - Wholemount, WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Nanog抗体
参阅全部 Nanog 一抗 -
描述
兔多克隆抗体to Nanog -
宿主
Rabbit -
经测试应用
适用于: ICC, ICC/IF, IHC-P, Flow Cyt, IHC - Wholemount, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80892于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (5) |
Use at an assay dependent concentration.
|
IHC-P | (5) |
Use at an assay dependent concentration.
|
Flow Cyt | (2) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC - Wholemount | (1) |
Use at an assay dependent concentration.
|
WB | (3) |
Use at an assay dependent concentration.
|
说明 |
---|
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC - Wholemount
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
组织特异性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
序列相似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
发展阶段
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 71950 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 6047 Mouse
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别名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (335)
ab80892 被引用在 335 文献中.
- Lee J et al. Production of large, defined genome modifications in rats by targeting rat embryonic stem cells. Stem Cell Reports 18:394-409 (2023). PubMed: 36525967
- Wakayama S et al. Healthy cloned offspring derived from freeze-dried somatic cells. Nat Commun 13:3666 (2022). PubMed: 35790715
- Xu M et al. Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit. Cell Prolif 55:e13209 (2022). PubMed: 35274380
- Yan S et al. CTGF Promotes the Osteoblast Differentiation of Human Periodontal Ligament Stem Cells by Positively Regulating BMP2/Smad Signal Transduction. Biomed Res Int 2022:2938015 (2022). PubMed: 36158888
- Song JH et al. Developmental Potency and Metabolic Traits of Extended Pluripotency Are Faithfully Transferred to Somatic Cells via Cell Fusion-Induced Reprogramming. Cells 11:N/A (2022). PubMed: 36291134