Anti-HP1 alpha抗体(ab77256)
Key features and details
- Goat polyclonal to HP1 alpha
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-HP1 alpha抗体
参阅全部 HP1 alpha 一抗 -
描述
山羊多克隆抗体to HP1 alpha -
宿主
Goat -
经测试应用
适用于: ICC/IF, Flow Cyt, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Cow, Dog, Rhesus monkey, Chinese hamster -
免疫原
Synthetic peptide:
C-NKRKSNFSNSADDIK
, corresponding to internal sequence amino acids 88-102 of Human HP1 alpha (NP_036249.1). -
阳性对照
- WB: A43N, K562 cell lysates. ICC/IF: U-2 OS cells. Flow Cyt: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab77256 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77256于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (4) |
Use a concentration of 10 µg/ml.
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
WB | (5) |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Primary incubation 1 hour at room temperature reccomended for this product. |
说明 |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Primary incubation 1 hour at room temperature reccomended for this product. |
靶标
-
功能
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
序列相似性
Contains 2 chromo domains. -
翻译后修饰
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
-
数据库链接
- Entrez Gene: 538885 Cow
- Entrez Gene: 477593 Dog
- Entrez Gene: 23468 Human
- Entrez Gene: 300266 Rat
- Omim: 604478 Human
- SwissProt: P45973 Human
- Unigene: 349283 Human
-
别名
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
图片
-
All lanes : Anti-HP1 alpha antibody (ab77256) at 0.03 µg/ml
Lane 1 : A43N cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling HP1 alpha with ab77256 at 10ug/ml for 1hr, followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
-
Flow cytometric analysis HeLa cells labeling HP1 alpha with ab77256 at 10 ug/mL (blue line). Cells were fixed with paraformaldehyde fixed and permeabilized with 0.5% Triton. Alexa Fluor 488 at 2ug/mL was used as the secondary antibody. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
实验方案
数据表及文件
-
Datasheet download
文献 (38)
ab77256 被引用在 38 文献中.
- Hsia CR et al. Confined migration induces heterochromatin formation and alters chromatin accessibility. iScience 25:104978 (2022). PubMed: 36117991
- Wang Q et al. The H2A.Z-KDM1A complex promotes tumorigenesis by localizing in the nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells. BMC Cancer 22:1166 (2022). PubMed: 36368958
- Noches V et al. Pilocarpine-induced seizures associate with modifications of LSD1/CoREST/HDAC1/2 epigenetic complex and repressive chromatin in mice hippocampus. Biochem Biophys Rep 25:100889 (2021). PubMed: 33426312
- Himeda CL et al. Targeted epigenetic repression by CRISPR/dSaCas9 suppresses pathogenic DUX4-fl expression in FSHD. Mol Ther Methods Clin Dev 20:298-311 (2021). PubMed: 33511244
- Kong NR et al. Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression. Cell Rep 34:108574 (2021). PubMed: 33406418