山羊抗小鼠IgG H&L (FITC) (ab6785)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB
Related conjugates and formulations
概述
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产品名称
山羊抗小鼠IgG H&L (FITC)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details -
免疫原
mouse whole molecule
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
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IHC (PFA fixed) |
1/1000.
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|
Immunomicroscopy |
Use at an assay dependent concentration.
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|
Flow Cyt |
1/500 - 1/2500. PubMed: 17989178
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|
IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
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|
ICC/IF | (1) |
1/1000 - 1/5000. (PMID16006199)
|
ELISA |
1/10000 - 1/50000.
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|
WB |
1/10000. PubMed: 17217624
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说明 |
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IHC (PFA fixed)
1/1000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/500 - 1/2500. PubMed: 17989178 |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365. |
ICC/IF
1/1000 - 1/5000. (PMID16006199) |
ELISA
1/10000 - 1/50000. |
WB
1/10000. PubMed: 17217624 |
图片
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ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (510)
ab6785 被引用在 510 文献中.
- Yu L et al. Extracellular vesicle-derived circCEBPZOS attenuates postmyocardial infarction remodeling by promoting angiogenesis via the miR-1178-3p/PDPK1 axis. Commun Biol 6:133 (2023). PubMed: 36726025
- Sarkis M et al. D2B-Functionalized Gold Nanoparticles: Promising Vehicles for Targeted Drug Delivery to Prostate Cancer. ACS Appl Bio Mater 6:819-827 (2023). PubMed: 36755401
- Li X et al. The members of the miR-148/152 family inhibit cancer stem cell-like properties in gastric cancer via negative regulation of ITGA5. J Transl Med 21:105 (2023). PubMed: 36765401
- Cai J et al. KDM4A, involved in the inflammatory and oxidative stress caused by traumatic brain injury-hemorrhagic shock, partly through the regulation of the microglia M1 polarization. BMC Neurosci 24:17 (2023). PubMed: 36869312
- Kaneko H et al. Zoledronate/Anti-VEGF Neutralizing Antibody Combination Administration Increases Osteal Macrophages in a Murine Model of MRONJ Stage 0-like Lesions. J Clin Med 12:N/A (2023). PubMed: 36902701