Apoptotic DNA Ladder分离试剂盒(ab65627)
Key features and details
- Assay type: Direct
- Sample type: Adherent cells, Suspension cells, Tissue
概述
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产品名称
Apoptotic DNA Ladder分离试剂盒
参阅全部 DNA fragmentation 试剂盒 -
样品类型
Tissue, Adherent cells, Suspension cells -
检测类型
Direct -
产品概述
Apoptotic DNA Ladder Isolation Kit (ab65627) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells and tissues. The new procedure selectively extracts DNA ladders without interference of intact genomic DNA in only 2-4 hours. As little as 5% of apoptotic cells in a sample can be visualized with this high sensitivity assay.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K170 Apoptotic DNA Ladder Isolation Kit. K170-50 is the same size as the 50 test size of ab65627.
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 50 tests Ammonium Acetate Solution 1 x 250µl DNA Ladder Extraction Buffer 1 x 12.5ml DNA Suspension Buffer 1 x 2ml Enzyme A Solution 1 x 250µl Enzyme B (Lyophilized) 1 vial -
研究领域
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相关性
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
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图片
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Apoptosis induced in Jurkat cells (1e6 cell x mL-1) by incubation for 24 hours with 1) Control medium; 2) 2 uM Camptothecin (ab120115); 3) 10 uM Camptothecin; 4) 10 uM Camptothecin + 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487); 5) 50 ng x mL-1 anti-Fas Ab (MBL), 6) 0.05 mM H2O2 (Sigma); 7) 1 mM H2O2. 5 uL of DNA preparation loaded into each well.
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DNA ladders were extracted from 2 x 106 Jurkat cells were treated with 2 μM Camptothecin. The kit extracts laddered DNA only but not intact genomic DNA; therefore, more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab65627 被引用在 6 文献中.
- Matsumoto S et al. Overexpressed exocyst complex component 3-like 1 spontaneously induces apoptosis. Biomed Res 42:109-113 (2021). PubMed: 34092752
- Okada J et al. TBC1D8B, a GTPase-activating protein, is a novel apoptosis inducer. Biomed Res 42:95-102 (2021). PubMed: 34092754
- Okada J et al. FAM83G Is a Novel Inducer of Apoptosis. Molecules 25:N/A (2020). PubMed: 32570757
- Aksoy E & Azkur AK Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner. J Vet Med Sci 81:204-212 (2019). PubMed: 30541984
- Yan MD et al. The anti-tumor activity of brown seaweed oligo-fucoidan via lncRNA expression modulation in HepG2 cells. Cytotechnology 71:363-374 (2019). PubMed: 30632031
- Mochizuki S et al. Effect of ADAM28 on carcinoma cell metastasis by cleavage of von Willebrand factor. J Natl Cancer Inst 104:906-22 (2012). Mouse . PubMed: 22636800