兔抗亚美尼亚仓鼠IgG H&L (HRP) (ab5745)
Key features and details
- Rabbit Anti-Armenian hamster IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, WB, ELISA, Electron Microscopy, IHC-P, ICC
概述
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产品名称
兔抗亚美尼亚仓鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Armenian hamster -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Armenian Hamster IgG and Armenian Hamster Serum. -
经测试应用
适用于: Dot blot, WB, ELISA, Electron Microscopy, IHC-P, ICCmore details -
免疫原
Armenian Hamster IgG whole molecule.
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. -
共轭说明
Horseradish Peroxidase (HRP). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
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Dot blot |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/5000.
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ELISA |
1/25000. This product has been assayed against 1.0 ug of Armenian Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
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Electron Microscopy |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/1000 - 1/5000.
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ICC |
1/1000 - 1/5000.
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说明 |
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Dot blot
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. |
ELISA
1/25000. This product has been assayed against 1.0 ug of Armenian Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. |
Electron Microscopy
Use at an assay dependent concentration. |
IHC-P
1/1000 - 1/5000. |
ICC
1/1000 - 1/5000. |
图片
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ab5745 tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (10)
ab5745 被引用在 10 文献中.
- Morimoto Y et al. Addiction of Merkel cell carcinoma to MUC1-C identifies a potential new target for treatment. Oncogene 41:3511-3523 (2022). PubMed: 35688945
- Fushimi A et al. Dependence on the MUC1-C Oncoprotein in Classic, Variant, and Non-neuroendocrine Small Cell Lung Cancer. Mol Cancer Res 20:1379-1390 (2022). PubMed: 35612556
- Li CG et al. Evodiamine Augments NLRP3 Inflammasome Activation and Anti-bacterial Responses Through Inducing a-Tubulin Acetylation. Front Pharmacol 10:290 (2019). PubMed: 30971927
- Boraschi-Diaz I et al. Collagen type I degradation fragments act through the collagen receptor LAIR-1 to provide a negative feedback for osteoclast formation. Bone 117:23-30 (2018). PubMed: 30217615
- Bouillez A et al. MUC1-C integrates PD-L1 induction with repression of immune effectors in non-small-cell lung cancer. Oncogene 36:4037-4046 (2017). PubMed: 28288138
- Bouillez A et al. MUC1-C promotes the suppressive immune microenvironment in non-small cell lung cancer. Oncoimmunology 6:e1338998 (2017). PubMed: 28932637
- Sofron A et al. High-resolution analysis of the murine MHC class II immunopeptidome. Eur J Immunol 46:319-28 (2016). PubMed: 26495903
- Bianchi R et al. Mutation of Threonine 34 in Mouse Podoplanin-Fc Reduces CLEC-2 Binding and Toxicity in Vivo While Retaining Anti-lymphangiogenic Activity. J Biol Chem 289:21016-21027 (2014). PubMed: 24907275
- Woessner DW & Lim CS Disrupting BCR-ABL in combination with secondary leukemia-specific pathways in CML cells leads to enhanced apoptosis and decreased proliferation. Mol Pharm 10:270-7 (2013). PubMed: 23211037
- Birjandi SZ et al. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol 186:3441-51 (2011). PubMed: 21307289