重组人Caspase-9蛋白(ab52203)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
描述
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产品名称
重组人Caspase-9蛋白
参阅全部 Caspase-9 蛋白酶 -
生物活性
Specific activity: 400 units/mg.
Unit definition: One unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab52203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 1089 Caspase-9, human recombinant. 1089-25 is the same size as the 25 unit size of ab52203.
UNIT DEFINITION: One unit of the recombinant Caspase 9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C.
常规信息
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别名
- APAF-3
- APAF3
- Apoptosis related cysteine peptidase
see all -
功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
组织特异性
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
发展阶段
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
翻译后修饰
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
图片
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Lane 1: Recombinant caspase 9 protein (Active) ab52203- 100 ng. Lane 2: BSA- 400 ng. Lane 3: BSA- 200 ng. Lane 4: BSA- 100 ng. Lane 5: BSA- 50 ng. Lane 6: BSA- 25 ng. SYPRO Ruby protein stain. Predicted band size: 46 kDa (precursor protein) and 35 kDa (p35 subunit) Observed Bands: 20 kDa and 11 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab52203 被引用在 2 文献中.
- Basu A et al. Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals. J Biol Chem 277:41850-6 (2002). PubMed: 12198125
- Azuma T et al. Gelsolin in complex with phosphatidylinositol 4,5-bisphosphate inhibits caspase-3 and -9 to retard apoptotic progression. J Biol Chem 275:3761-6 (2000). PubMed: 10660524